In vitro and in vivo validation of ligA and tarI as essential targets in Staphylococcus aureus.
نویسندگان
چکیده
A conditional expression system has been developed using the isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible Pspac promoter to validate essential genes of Staphylococcus aureus in vivo. The system has been applied to prove the essentiality of ligA and to evaluate the function of tarI, which was found to be essential in vitro but not in vivo.
منابع مشابه
In-vitro and In-vivo Evaluation of Silymarin Nanoliposomes against Isolated Methicillin-resistant Staphylococcus aureus
Staphylococcus aureus is an opportunistic pathogen and remains a common cause of burn wound infections. Different studies have shown that entrapment of plant-derived compounds into liposomes could increase their anti-Staphylococcus aureus activity. Silymarin is the bioactive extract from the known plant Silybum marianum L. The objective of this study was to evaluate efficacy of silymarin in fre...
متن کاملIn-vitro and In-vivo Evaluation of Silymarin Nanoliposomes against Isolated Methicillin-resistant Staphylococcus aureus
Staphylococcus aureus is an opportunistic pathogen and remains a common cause of burn wound infections. Different studies have shown that entrapment of plant-derived compounds into liposomes could increase their anti-Staphylococcus aureus activity. Silymarin is the bioactive extract from the known plant Silybum marianum L. The objective of this study was to evaluate efficacy of silymarin in fre...
متن کاملEvaluation of γ-irradiation treatment on the antibacterial activities of Mentha piperita L. essential oils in vitro and in vivo systems (CLP inflammatory model)
Background: Mentha piperita L. essential oils have different antibacterial activity. In the present study, we investigated the effect of &gamma–irradiation on the antibacterial activities of Mentha piperita L. essential oils in vitro and in vivo systems. Materials and Methods: The aerial parts of peppermint were irradiated in a cobalt60 source with 0, 10 and 25 kGy absorbed doses. Then, t...
متن کاملAntimicrobial Effect of Zataria Essential Oil on the Skin Bacteria in Wistar Rats
Objective Zataria is one of the native plants of Iran which is widely used for the treatment of diseases among Iranians. In this study, we investigated the antimicrobial effects of Zataria essential oil on the skin bacteria in rats. Methods Bacterial strains were isolated from the skin of 6 wistar rats and the antimicrobial effects of Zataria essential oil were evaluated by disk diffusion and ...
متن کاملEvaluation of Antibacterial Effects of Lecanora muralis SP. Extract on Staphylococcus Aureus and Salmonella Typhimurium in in-vitro and in animal model
Background & Objective: Staphylococcus aureus and Salmonella typhimurium are among important causes of infection and antibiotic resistance. It is essential to find antimicrobial compounds against them. The objective of this study is to evaluate the antibacterial activity of Lecanora muralis SP. extracts on Staphylococcus aureus and Salmonella typhimurium in in-vitro and in animal model.Ma...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 52 12 شماره
صفحات -
تاریخ انتشار 2008